EXCYTEX has ample experience and know-how of state-of-the-art techniques to produce (targeted) lipid bilayer vesicles. EXCYTEX creates value by offering this expertise to labs which do not have this knowledge or equipment. By combining the latest findings in the field of extracellular vesicles with the experience in the field of liposomes, EXCYTEX will develop a set of research tools that provides a solution to several much needed issues that scientists encounter in their daily operations.

1. XCX-MIMIC

XCX -MIMIC encompasses our liposome-based product line. Liposomes are vesicles, composed of a lipid bilayer that resembles the basic outer structure of extracellular vesicles. When combined with specific proteins and nucleic acids, these liposomes can be tailored to mimic several important characteristics of extracellular vesicles.

XCXmimicThey will also have added functionalities, thereby strengthening their applicability as a reference tool in the lab, for example by adding a fluorescent lipid to the vesicle composition or as a soluble protein. Simple mimics contain 100 nm vesicles.  Lipid compositions can be customised, but in general the double lipid membrane of vesicles consist of sphingomyelin, phosphatidylserine, phosphatidylcholine, phosphatidylethanolamide, phosphatidylinositol and cholesterol. More specialised mimics, encompass proteins and nucleic acid, in combination with a fluorescent label. The high-end products in this product line contain specific quantities of certain proteins and RNA species that mimic distinct sub-groups of extracellular vesicles and can be combined with different sizes.

 

2. XCX-TARGET

XCX-TARGET contains our targeting techniques. By adding antibody linkers or post insertion techniques to our XCX-mimics, it is possible to direct them to certain targets. These vesicles can be used for reference studies, capturing and targeting. Fluorescent antibodies can be used to create a standard range of different fluorescent intensities. Furthermore we are working on a kit that can be used to functionalize exosomes of biological origins.

XCX-mimic2

Other options to functionalize XCX-mimic is to combine 1) biotin to follow the fate of vesicles (with streptavidin), 2) deferoxiamine to label the vesicles (with radioactive metals), 3) TAT-peptides to make them fusogenic, 4) fluorescent group to label vesicles and 5) inert polymers that inhibits interaction with non-target cells.

Idealy we would be able to create a synthethic exosome that perfectly resembles a biological exosome. However several qualities are too expensive or too dificult to mimic. EXCYTEX pays close attention to further developments in the field of extracellular vesicles as well as liposomes to provide state of the art solutions. If you have any questions or remarks please contact us via the contact page.